Evidence of starch accumulation in TBY-2 cells in the presence of auxin

Tobacco cell suspension (TBY-2) is known to produce starch when cultured in medium supplemented with cytokinin or hormone-free medium. Unexpectedly, TBY-2 cells, continuously cultivated on auxin alone, were also able accumulate at the beginning of stationary growth phase a yield 9.22 ± 0.68 percent. This production was strongly correlated 25-fold increase synthase activity. Moreover, this line an amylopectin-rich ratio amylopectin over amylose 2.7 which linked typical small granules (size around 1.4 µm). According our preliminary results, plant could low-cost rich needed food industry for edible film bioplastic without impacts from climate season changes.